PHYB/Anti-PhytochromeBantibodyProductInformation  Description:Mousemonoclonal(Clone:10P5)antibody  Background:PHYBisared/far-redphotoreceptorinvolvedintheregulationofde-etiolation.Itexistsintwointer-convertibleforms:PrandPfr(active).Itisinvolvedinthelight-promotionofseedgerminationandintheshade*oidanceresponse. Synonyms:PHYB,HY3,OOP1,OUTOFPHASE1,PHYTOCHROMEB  Immunogen:RecombinantproteinofPHYBderivedfrom Arabidopsisthaliana AT2G18790.  Form:Lyophilized Quantity:150 g Purification:ProteinApurified  Reconstitution:  Reconstitutionwith150lof0.01MsterilePBS. "Note:pleasespintubebrieflypriortoopeningitto*oidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetube".  Stability&    Storage:Useamanualdefrostfreezerand*oidrepeatedfreeze-thawcycles. 12monthsfromdateofreceipt,-20to-70℃assupplied.6months,-20to-70℃understerileconditionsafterreconstitution. 1month,2to8℃understerileconditionsafterreconstitution. Shipping:Theproductisshippedat4℃.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedabove.ApplicationInformation  Applications: WesternBlot(1:1000-1:2000) Note:Optimaldilutions/conce*ationsshouldbedeterminedbytheenduser.  ExpectedResults: 125kDa  Confirmed Reactivity: Arabidopsisthaliana Predicted Reactivity: Formorespecieshomologuesinformation,pleasecontacttechsupportattech@phytoab.com.ApplicationExampleLane1:35μgtotalproteinfrom phyB-9 mutantof Arabidopsisthaliana leaf.Lane2:35μgtotalproteinfrom Arabidopsisthaliana leaf. Electrophoresis: 8%SDS-Urea-PAGETransfer: blottingtoNC(nitrocellulose)membranefor1h.Blocking: 5%skimmilkatRTor4℃for1h.Primaryantibody: 1:1000dilutionovernightat4℃.Secondaryantibody: 1:5000dilutionusingGoatAnti-MouseIgGH&L(HRP).Detection: usingchemiluminescencesubstrateandimagewerecapturedwithCCDcamera.标签：光敏色素 光敏色素 北京市phyb光敏色素 北京市phyb光敏色素厂家[光敏色素] 北京市phyb光敏色素厂家PHYB/光敏色素